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Would recommend retesting on a new specimen at a later date electrophoresis migration of charged particles or solutes in an electrical field five fractions albumin, alpha 1, alpha 2, beta, and gamma Gamma fraction where immunoglobulins hang out electrophoresis use detects immunoglobulin disorders monoclonal and polyclonal gammopathy 2 types of immunoglobulin disorders Monoclonal one clone of a plasma cell makes too much Ig of a single class monoclonal protein, M protein, or para protein Other names for monoclonal gammopathy Polyclonal several clones of plasma cells make too much of two or more classes of Ig secondary condition usually polyclonal gammopathies fall into this category IEP immunoelectrophoresis Older immunoelectrophoresis serum or urine IEP can be done on 1st phase of IEP electrophoresis of control and patient 2nd phase of IEP Double immunodiffusion; specific antisera against heavy and light chains are placed in troughs to separate samples IEP immunodiffusion IgG, IgA, IgM, kappa, and lambda 18-48 hrs time needed for immunodiffusion stop 2nd phase IEP rinse with isotonic saline shape, intensity, and location staining allows you to compare these things of arcs in 2nd phase Applications of IEP determines elevation or deficiency in Ig and determines Ig structure IFE Immunofixation electrophoresis Serum, urine, CSF, and other body fluids IFE can be done on 1st phase IFE Electrophoresis 2nd phase IFE 5 thin strips of paper soaked in monospecific antisera are applied to the top of each trough and 1 with a control solution in the 6th trough; antisera diffuses directly into the sample and antibody-antigen complexes are fixed in the gel which can be stained IFE incubation dramatically shortened to less than an hour IFE obbjectives identifies nature of band; classifies the isotype of the monoclonal gammopathy, identifies smaller monoclonal gammopathy intact, free chain, mix identifies that nature of the band IgG, IgM, etc Classifies the isotype of the monoclonal gammopathy Negative IFE bands are a blush or a smear Hypogammaglobulinemia Low amount; faintly stained bands Polyclonal gammopathy many isotypes; darkly staining broad bands in the gamma region Monoclonal gammopathy one isotype; dark and narrow bands in specific lanes Prozoning area of antigen excess that results in burnt out appearance in the center Dilute How to fix prozoning if seen in electrophoresis Pro to IEP Technically simpler and cheaper; less subject to antigen excess; better for large monoclonal gammopathy CON to IEP Relatively insensitive; difficult to interpret; long incubation time PRO to IFE Greater sensitivity and resolution; can detect smaller bands and earlier stage of monoclonal gammopathies; easier to interpret; shorter incubation time; more specimen options CON to IFE dilutions may be necessary to avoid prozoning YOU MIGHT ALSO LIKE.QuizletproductClickLinkData name:CHEM 2001 Exam IV,id:16475653,price:,category:premium content,variant:study guide,position:,brand:lerwin3; QLoad(Quizlet.productClickLinkData); return;)elsewindow.QuizletproductClickLinkData name:CHEM 2001 Exam IV,id:16475653,price:,category:premium content,variant:study guide,position:,brand:lerwin3; QLoad(Quizlet.productClickLinkData); return;;window.location.assign( ); id1-16475653 CHEM 2001 Exam IV 80 terms lerwin3 window.QuizletproductClickLinkData name:Thin Layer Chromatography,id:41900272,price:,category:premium content,variant:study guide,position:,brand:scarlson4; QLoad(Quizlet.productClickLinkData); return;)elsewindow.QuizletproductClickLinkData name:Thin Layer Chromatography,id:41900272,price:,category:premium content,variant:study guide,position:,brand:scarlson4; 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